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NCT06342414 | RECRUITING | Hepatocellular Carcinoma


An Exosome-Based Liquid Biopsy for the Differential Diagnosis of Primary Liver Cancer
Sponsor:

City of Hope Medical Center

Brief Summary:

It is sometimes difficult to precisely understand whether a primary liver cancer is a hepatocellular carcinoma or a cholangiocarcinoma. The researchers will develop and validate a liquid biopsy, based on exosomal content analysis and powered by machine learning, to help clinicians differentiate these two cancers before surgery.

Condition or disease

Hepatocellular Carcinoma

Intrahepatic Cholangiocarcinoma

Cholangiocarcinoma

Primary Liver Cancer

Primary Liver Carcinoma

Hepatic Cancer

Hepatic Carcinoma

Intervention/treatment

ELUCIDATE

Detailed Description:

Primary liver cancers (PLCs) encompass a diverse group of malignancies originating from the liver, collectively ranking as the third leading cause of cancer-related mortality worldwide in 2020. Among PLCs, intrahepatic cholangiocarcinoma (ICC) and hepatocellular carcinoma (HCC) represent the most predominant subtypes. Despite their collective grouping as PLCs, ICC and HCC patients exhibit distinct etiologies, pathologies, and clinical characteristics, necessitating different treatment approaches. Accurate differentiation between ICC and HCC is paramount to optimize patient outcomes and guide personalized treatment decisions. However, a definitive diagnosis is often obtained only after the pathological review of the resected neoplastic tissue, which requires invasive tumor sampling and poses risks of complications such as hemorrhage and tumor cell seeding. Consequently, there is a pressing clinical need to develop noninvasive diagnostic approaches to achieve an accurate differential diagnosis for patients with these distinct forms of PLCs. This study involves the development and validation of a liquid biopsy, assessing circulating exosomal microRNAs (exo-miRNA) for indirect sampling of tumor tissue in the bloodstream. The researchers intend to harness machine learning and bioinformatics to create a cost-efficient, non-invasive, clinic-friendly assay with high sensitivity and specificity, aiding the differential diagnosis between ICC and HCC. The researchers intend to do so in three phases: 1. To perform comprehensive small RNA-Seq from exo-miRNA from patients with ICC and HCC. 2. To develop and train a differential diagnosis panel based on advanced machine-learning models to obtain a final differential diagnosis biomarker. 3. To validate the findings in an independent cohort of ICC and HCC. In summary, this proposal promises to improve patient care and help clinicians perform a more reliable differential diagnosis between ICC and HCC in patients with primary liver cancer.

Study Type : OBSERVATIONAL
Estimated Enrollment : 400 participants
Official Title : An Exosome-Based Liquid Biopsy for the Differential Diagnosis of Primary Liver Cancer
Actual Study Start Date : 2024-03-15
Estimated Primary Completion Date : 2026-06-18
Estimated Study Completion Date : 2026-06-18

Information not available for Arms and Intervention/treatment

Ages Eligible for Study: 18 Years
Sexes Eligible for Study: ALL
Accepts Healthy Volunteers:
Criteria
Inclusion Criteria
  • * A histologically confirmed diagnosis of hepatocellular carcinoma
  • * A histologically confirmed diagnosis of intrahepatic cholangiocarcinoma
  • * Received standard diagnostic and staging procedures as per local guidelines
  • * Availability of at least one blood-derived sample, drawn before receiving any curative-intent treatment
Exclusion Criteria
  • * Lack of or inability to provide informed consent
  • * Synchronous hepatocellular carcinoma and intrahepatic cholangiocarcinoma
  • * Primary liver cancer other than hepatocellular carcinoma or intrahepatic cholangiocarcinoma
  • * Secondary liver cancer

An Exosome-Based Liquid Biopsy for the Differential Diagnosis of Primary Liver Cancer

Location Details

NCT06342414


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Locations


RECRUITING

United States, California

City of Hope Medical Center

Duarte, California, United States, 91010

RECRUITING

Japan,

Graduate School of Medical Sciences, Kyushu University

Fukuoka, Japan,

RECRUITING

Japan,

Graduate School of Medical Sciences, Kumamoto University

Kumamoto, Japan,

RECRUITING

Japan,

Hokkaido University Graduate School of Medicine

Sapporo, Japan,

RECRUITING

Japan,

Tokushima University

Tokushima, Japan,

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