Sohag University
Zaghloul Amin medicine
Evaluation of serum leptin in vitiligo patients and control Assessing its correlation to the body mass index and disease dermographic data .
Vitiligo
serum leptin
Not Applicable
, leptin is supposed to contribute to vitiligo pathogenesis through promoting the release of pro-inflammatory mediators such as tumor necrosis factor α "TNF," IL-2 and IL-6, as well as enhancing cytotoxic T cells "CD8" recruitment and inhibiting the functions of regulatory T cells "Tregs". Leptin can also aid in induction of oxidative stress by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase• Venous blood samples will be taken from patients and controls to evaluate serum leptin: Morning samples will be taken after 12-h overnight fast in plain vacutainer tubes and directly centrifuged (1098 g for 15 min). Sera will be then collected in plain, capped, 5 ml centrifuge tubes "Eppendorf" and freezed at -20ᵒC in a well-monitored freezer until the analysis is performed• Serum leptin will be quantitatively assessed in vitro using DRG leptin Sandwich Enzyme Linked Immuno-Sorbent Assay "ELISA". ELISA will be performed according to manufacturer's guidelines where each run included a standard curve. Using the mean absorbance value for each sample, the corresponding concentration from the standard curve will be determined.}}
Study Type : | Interventional |
Estimated Enrollment : | 400 participants |
Masking : | None (Open Label) |
Primary Purpose : | Treatment |
Official Title : | Assessment of Serum Leptin in Vitiligo Patients |
Actual Study Start Date : | November 2022 |
Estimated Primary Completion Date : | September 2023 |
Estimated Study Completion Date : | September 2023 |
Arm | Intervention/treatment |
---|---|
Active Comparator: group A obese with vitiligo |
Diagnostic Test: serum leptin |
Active Comparator: group b obese without vitiligo |
Diagnostic Test: serum leptin |
Active Comparator: group C non obese with vitiligo |
Diagnostic Test: serum leptin |
Active Comparator: group D non obese without vitiligo |
Diagnostic Test: serum leptin |
Ages Eligible for Study: | 18 Years |
Sexes Eligible for Study: | All |
Accepts Healthy Volunteers: | No |
Want to participate in this study, select a site at your convenience, send yourself email to get contact details and prescreening steps.
Not yet recruiting
Sohag University Hospital
Sohag, Egypt,