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NCT02252588 | Completed | Chronic Obstructive Pulmonary Disease


The Effect of Chlorhexidine on the Oral and Lung Microbiota in Chronic Obstructive Pulmonary Disease
Sponsor:

University of Minnesota

Brief Summary:

Determine the effect of twice-daily chlorhexidine oral rinse on oral and lung microbiota biomass in subjects with chronic obstructive pulmonary disease (COPD) with chronic bronchitis. Our primary outcome will be to compare the microbiota biomass (number of bacteria as measured by 16S rRNA copy number) of induced sputum and the oral cavity before and after 8 weeks of twice-daily chlorhexidine oral rinse (n=25) compared to controls (n=25) using qPCR and next-generation sequencing of the bacterial 16S rRNA gene comparing total bacterial biomass

Condition or disease

Chronic Obstructive Pulmonary Disease

Intervention/treatment

Chlorhexidine

Placebo

Phase

Phase 2

Detailed Description:

Our hypothesis is that 8 weeks of chlorhexidine oral rinse will decrease microbiota biomass compared to baseline and those on placebo. Furthermore, we hypothesize that chlorhexidine treatment will: i) decrease lung and oral microbiota diversity; ii) alter microbiota taxonomic composition in the lung and oral cavity; iii) decrease systemic inflammation as measured by blood high sensitivity C-reactive protein (hsCRP), fibrinogen and leukocyte count; and iv) demonstrate a trend towards improvement in respiratory health status as measured by the Breathlessness, Cough, and Sputum Scale (BCSS)[1, 2] and St. George's Respiratory Questionnaire (SGRQ). Subaim 1: Determine if chlorhexidine alters the lung and oral rinse microbiota diversity and taxonomic composition. Our hypothesis is that chlorhexidine oral rinse will decrease the diversity (Shannon and inverse Simpson diversity indices) and taxonomic composition of both oral and lung microbiota compared to those on placebo as determined by next-generation sequencing of the bacterial 16S rRNA gene. Subaim 2: Determine the impact of chlorhexidine on systemic inflammation. Our hypothesis is that the decrease in lung microbiota biomass is associated with a decrease in systemic inflammation as measured by blood hsCRP, fibrinogen, and leukocyte count. Subaim 3: Determine if respiratory symptoms associate with the lung microbiota biomass. Our hypothesis is that chlorhexidine will demonstrate improved respiratory health status as measured by the BCSS and SGRQ.

Study Type : Interventional
Estimated Enrollment : 44 participants
Masking: Double
Primary Purpose: Basic Science
Official Title: The Effect of Chlorhexidine on the Oral and Lung Microbiota in Chronic Obstructive
Actual Study Start Date : September 1, 2014
Estimated Primary Completion Date : December 15, 2019
Estimated Study Completion Date : January 1, 2020
Arm Intervention/treatment

Experimental: Chlorhexidine

Oral Rinse

Drug: Chlorhexidine

Other: Placebo

Placebo Comparator: Placebo

Oral Rinse

Drug: Chlorhexidine

Other: Placebo

Ages Eligible for Study: 40 Years to 85 Years
Sexes Eligible for Study: All
Accepts Healthy Volunteers: No
Criteria
Inclusion Criteria
  • Willingness to undergo sputum induction
  • Capability to provide written informed consent
  • Age ≥ 40 years and ≤ 85 years
  • FEV1/FVC ratio (post bronchodilator) ≤70%
  • FEV1 (post bronchodilator) ≤ 65%
  • Presence or high likelihood of chronic cough and sputum production defined as one of the following
    • Presence of chronic cough and sputum will be defined by responses to the first two questions on the SGRQ. Subjects who respond positively to both question 1 (cough) and question 2 (sputum) on the SGRQ as either "several days per week" or "almost every day" will be eligible.
    • COPD exacerbation within the previous 12 months defined as taking antibiotics and/or prednisone for respiratory symptoms, hospitalization or emergency department visit for respiratory illness.
    • Current or former smoker with lifetime cigarette consumption of > 10 pack-years
    • Negative serum pregnancy test at the baseline visit if patient is a pre-menopausal female (menopause defined as absence of a menstrual cycle in the last 12 months)
    • Must be fluent in speaking the English language
    • Have a minimum of four teeth
    Exclusion Criteria
    • Not fully recovered for at least 30 days from a COPD exacerbation.
    • Treated with antibiotics in the last 2 months.
    • The presence of dentures (full plate).
    • Active oral infection being treated by health care professional.
    • Current use of chlorhexidine or over-the-counter mouth washes in the last 2 months.
    • Known allergy or sensitivity to chlorhexidine
    • Unstable cardiac disease
    • Clinical diagnosis of asthma, bronchiectasis, cystic fibrosis, or severe alpha-1 antitrypsin deficiency
    • Active lung cancer or history of lung cancer if it has been less than 2 years since lung resection or other treatment. If history of lung cancer, must have no evidence of recurrence in the 2 years preceding the baseline visit.

The Effect of Chlorhexidine on the Oral and Lung Microbiota in Chronic Obstructive Pulmonary Disease

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The Effect of Chlorhexidine on the Oral and Lung Microbiota in Chronic Obstructive Pulmonary Disease

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Locations


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United States, Minnesota

VA Medical Center

Minneapolis, Minnesota, United States, 55417

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